ABSTRACT
Objective:To explore the potential molecular targets and mechanism of Zeqi Decoction in the treatment of lung adenocarcinoma (LUAD) through bioinformatics and cell experiment.Methods:The active components of Zeqi Decoction were collected based on TCMSP database and literature search. Then R software was used to screen differentially expressed genes in LUAD from TCGA and GEO databases. The co-expression module was obtained through weighted gene co-expression network analysis (WGCNA), and the potential targets were obtained after matching and mapping with targets of Zeqi Decoction. Enrichment analysis of GO function and KEGG pathway of targets was conducted. The results were experimentally verified. The lung adenocarcinoma cell lines A549 and H1299 were divided into blank control group and Zeqi Decoction group according to random number table. The inhibition rate of cell proliferation was detected by cell proliferation test (CCK-8); the expression of leukocyte differentiation antigen 36 (CD36) in A549 and H1299 cells was detected by Western blot; the levels of low density lipoprotein receptor (LDLR) and IL-6 were detected by ELISA.Results:Totally 157 anti-lung adenocarcinoma active components and 18 potential targets were obtained, mainly including CD36, IL6, LDLR, etc. The main target of Zeqi Decoction in the treatment of lung adenocarcinoma was lipid metabolism. The results showed that Zeqi Decoction could effectively inhibit the activity of A549 and H1299 cells and the levels of CD36, LDLR and IL-6.Conclusion:Zeqi Decoction can inhibit the inflammatory response by down-regulating the protein expressions of CD36 and LDLR, thereby slowing the proliferation of cells.
ABSTRACT
Objective@#To explore the effect of early pulmonary rehabilitation on stroke associated pneumonia by the ICU specialist nurses.@*Methods@#Totally 40 cases of stroke patients from January 2017 to June 2017 were selected into the control group, and 44 cases of stroke patients from July 2017 to December 2017 were set as the intervention group. The patients in the control group were given routine care, and those in the experimental group were given early pulmonary rehabilitation in addition to routine care by ICU specialist nurses. The clinical outcome such as the occurrence of SAP, the length of intensive care units (ICU) stay and the duration of mechanical ventilation were compared.@*Results@#The incidence of SAP in the intervention group was 13.64%(6/44), significantly lower than that 32.50(13/40) in the control group (χ2=4.26, P<0. 05), and the length of ICU stay the duration of mechanical ventilation were (5.59±3.93), (3.2±0.84)days, which were lower than (8.50±7.89), ((13.13±9.58)days in the control group (t=2.106,2.678, P<0.05) , the prealbumin levels was (219.43±59.71) mg/L,higher than (192.20±54.85) mg/L in the control group (t=-2.170, P<0.05) , the difference was statistically significant.@*Conclusion@#Early pulmonary rehabilitation can effectively reduce the incidence of SAP and better clinical outcome. And we also should improve the SAP risk assessment and the management of swallowing and nutrition.
ABSTRACT
Objective To explore the effect of early pulmonary rehabilitation on stroke associated pneumonia by the ICU specialist nurses. Methods Totally 40 cases of stroke patients from January 2017 to June 2017 were selected into the control group, and 44 cases of stroke patients from July 2017 to December 2017 were set as the intervention group. The patients in the control group were given routine care, and those in the experimental group were given early pulmonary rehabilitation in addition to routine care by ICU specialist nurses. The clinical outcome such as the occurrence of SAP, the length of intensive care units (ICU) stay and the duration of mechanical ventilation were compared. Results The incidence of SAP in the intervention group was 13.64% (6/44), significantly lower than that 32.50(13/40) in the control group ( χ2=4.26﹐P＜0. 05)﹐and the length of ICU stay the duration of mechanical ventilation were (5.59±3.93), (3.2±0.84)days﹐which were lower than (8.50±7.89),((13.13±9.58)days in the control group (t=2.106,2.678﹐P＜0.05), the prealbumin levels was(219.43±59.71)mg/L,higher than(192.20±54.85) mg/L in the control group(t=-2.170﹐P＜0.05), the difference was statistically significant. Conclusion Early pulmonary rehabilitation can effectively reduce the incidence of SAP and better clinical outcome. And we also should improve the SAP risk assessment and the management of swallowing and nutrition.
ABSTRACT
Objective To study the changes of oral flora in patients with diabetic ketoacidosis,in order to provide evidence for making oral nursing intervention and hygiene education. Methods 35 patients with diabetic ketoacidosis were named as group A,35 non-diabetic patients with chronic periodontitis (diagnostic criteria:periodontal pocket 14 mm) were named as group B,35 nonketotic patients with diabetes mellitus were named as group C,then all the patients were detected for the oral disease and the oral hygiene was evaluated. Results The gingival index,plaque index,tooth mobility,probing depth and hemorrhage after the detection of three groups had no significant differences. The detection rate of streptococcus oralis, lactobacillus,fusobacterium nucleatum,black-pigment bacteria,Capnocytophaga gingivalis,actinomycetes, escherichia coli,staphylococcus aureus and pseudomonas aeruginosa had no significant differences,there was a positive correlation between quantity of black-pigment bacteria, Capnocytophaga gingivalis and fasting blood glucose and glycosylated hemoglobin of patients with diabetic ketoacidosis. Conclusions Diabetic ketoacidosis strengthened the bacterial invasion and oral colonization of patients.